6*His, His-Tag antibody - 100 µg
Host : Mouse
Clonality: Monoclonal
Clone: 1C4
Isotype: IgG1
Immunogen:
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight:
Uniprot:
Gene id:
Background: Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.
Field of research:
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, WB, IP, IF
Dilution: WB: 1:1000-1:10000; IP: 1:1000-1:10000; IF: 1:20-1:100
Target: 6*His, His-Tag
Purification: Protein A+G purification
Reactivity: Recombinant protein
Clonality: Monoclonal
Clone: 1C4
Isotype: IgG1
Immunogen:
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight:
Uniprot:
Gene id:
Background: Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.
Field of research:
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, WB, IP, IF
Dilution: WB: 1:1000-1:10000; IP: 1:1000-1:10000; IF: 1:20-1:100
Target: 6*His, His-Tag
Purification: Protein A+G purification
Reactivity: Recombinant protein