TDG antibody - 100 µg
Host : Rabbit
Clonality: Polyclonal
Clone:
Isotype: IgG
Immunogen: thymine-DNA glycosylase
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight: 55-60 kDa
Uniprot: Q13569
Gene id: 6996
Background: DNA glycosylase that plays a key role in active DNA demethylation: specifically recognizes and binds 5-formylcytosine(5fC) and 5-carboxylcytosine(5caC) in the context of CpG sites and mediates their excision through base-excision repair(BER) to install an unmethylated cytosine. Cannot remove 5-hydroxymethylcytosine(5hmC). According to an alternative model, involved in DNA demethylation by mediating DNA glycolase activity toward 5-hydroxymethyluracil(5hmU) produced by deamination of 5hmC. Also involved in DNA repair by acting as a thymine-DNA glycosylase that mediates correction of G
T mispairs to G
C pairs: in the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G
T mismatches. Its role in the repair of canonical base damage is however minor compared to its role in DNA demethylation. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G
T, it can remove thymine also from C
T and T
T mispairs in the order G
T >> C
T > T
T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A
T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.
Field of research: Metabolism
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, WB, IP
Dilution: WB: 1:500-1:2000; IP: 1:200-1:2000
Target: TDG
Purification: Immunogen affinity purified
Reactivity: Human, Mouse, Rat
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Clonality: Polyclonal
Clone:
Isotype: IgG
Immunogen: thymine-DNA glycosylase
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight: 55-60 kDa
Uniprot: Q13569
Gene id: 6996
Background: DNA glycosylase that plays a key role in active DNA demethylation: specifically recognizes and binds 5-formylcytosine(5fC) and 5-carboxylcytosine(5caC) in the context of CpG sites and mediates their excision through base-excision repair(BER) to install an unmethylated cytosine. Cannot remove 5-hydroxymethylcytosine(5hmC). According to an alternative model, involved in DNA demethylation by mediating DNA glycolase activity toward 5-hydroxymethyluracil(5hmU) produced by deamination of 5hmC. Also involved in DNA repair by acting as a thymine-DNA glycosylase that mediates correction of G
T mispairs to G
C pairs: in the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G
T mismatches. Its role in the repair of canonical base damage is however minor compared to its role in DNA demethylation. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G
T, it can remove thymine also from C
T and T
T mispairs in the order G
T >> C
T > T
T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A
T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.
Field of research: Metabolism
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, WB, IP
Dilution: WB: 1:500-1:2000; IP: 1:200-1:2000
Target: TDG
Purification: Immunogen affinity purified
Reactivity: Human, Mouse, Rat