RAF1 antibody - 100 µg
Host : Rabbit
Clonality: Polyclonal
Clone:
Isotype: IgG
Immunogen: v-raf-1 murine leukemia viral oncogene homolog 1
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight: 76 kDa
Uniprot: P04049
Gene id: 5692
Background: Serine
threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK
ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase(MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases(MAP2K1
MEK1 and MAP2K2
MEK2) and the extracellular signal-regulated kinases(MAPK3
ERK1 and MAPK1
ERK2). The phosphorylated form of RAF1(on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD
Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2
cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility(ROCK2), apoptosis(MAP3K5
ASK1 and STK3
MST2), proliferation and angiogenesis(RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD
Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin(OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2
SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.
Field of research: Cancer, Immunology, Cardiovascular, Signal Transduction
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, WB, IHC
Dilution: WB: 1:500-1:2000; IHC: 1:20-1:200
Target: RAF1
Purification: Immunogen affinity purified
Reactivity: Human, Mouse
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Clonality: Polyclonal
Clone:
Isotype: IgG
Immunogen: v-raf-1 murine leukemia viral oncogene homolog 1
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight: 76 kDa
Uniprot: P04049
Gene id: 5692
Background: Serine
threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK
ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase(MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases(MAP2K1
MEK1 and MAP2K2
MEK2) and the extracellular signal-regulated kinases(MAPK3
ERK1 and MAPK1
ERK2). The phosphorylated form of RAF1(on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD
Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2
cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility(ROCK2), apoptosis(MAP3K5
ASK1 and STK3
MST2), proliferation and angiogenesis(RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD
Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin(OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2
SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.
Field of research: Cancer, Immunology, Cardiovascular, Signal Transduction
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, WB, IHC
Dilution: WB: 1:500-1:2000; IHC: 1:20-1:200
Target: RAF1
Purification: Immunogen affinity purified
Reactivity: Human, Mouse