PHF2 antibody - 100 µg
Host : Rabbit
Clonality: Polyclonal
Clone:
Isotype: IgG
Immunogen: PHD finger protein 2
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight: 100 kDa, 150 kDa
Uniprot: O75151
Gene id: 5253
Background: Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3(H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3(H3K4me3) at rDNA promoters and promotes expression of rDNA.
Field of research: Epigenetics, Metabolism
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, WB, IHC, IP
Dilution: WB: 1:200-1:1000; IP: 1:500-1:1000; IHC: 1:20-1:200
Target: PHF2
Purification: Immunogen affinity purified
Reactivity: Human
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Clonality: Polyclonal
Clone:
Isotype: IgG
Immunogen: PHD finger protein 2
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight: 100 kDa, 150 kDa
Uniprot: O75151
Gene id: 5253
Background: Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3(H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3(H3K4me3) at rDNA promoters and promotes expression of rDNA.
Field of research: Epigenetics, Metabolism
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, WB, IHC, IP
Dilution: WB: 1:200-1:1000; IP: 1:500-1:1000; IHC: 1:20-1:200
Target: PHF2
Purification: Immunogen affinity purified
Reactivity: Human