MBP tag antibody - 100 µg
Host : Mouse
Clonality: Monoclonal
Clone: 0D8
Isotype: IgG2a
Immunogen: MBP-Tag
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight: 40 kDa
Uniprot:
Gene id:
Background: Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin.
Field of research:
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, WB, IP
Dilution: WB: 1:1000-1:8000; IP: 1:5000-1:50000
Target: MBP tag
Purification: Protein A+G purification
Reactivity: Recombinant protein
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Clonality: Monoclonal
Clone: 0D8
Isotype: IgG2a
Immunogen: MBP-Tag
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight: 40 kDa
Uniprot:
Gene id:
Background: Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin.
Field of research:
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, WB, IP
Dilution: WB: 1:1000-1:8000; IP: 1:5000-1:50000
Target: MBP tag
Purification: Protein A+G purification
Reactivity: Recombinant protein