Histone H3.3 antibody - 100 µg
Host : Rabbit
Clonality: Polyclonal
Clone:
Isotype: IgG
Immunogen: H3 histone, family 3B(H3.3B)
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight: 17 kDa
Uniprot: P84243
Gene id: 3021
Background: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Field of research: Epigenetics, Metabolism
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, WB
Dilution: WB: 1:500-1:2000
Target: Histone H3.3
Purification: Immunogen affinity purified
Reactivity: Human, Mouse, Rat
Clonality: Polyclonal
Clone:
Isotype: IgG
Immunogen: H3 histone, family 3B(H3.3B)
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight: 17 kDa
Uniprot: P84243
Gene id: 3021
Background: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Field of research: Epigenetics, Metabolism
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, WB
Dilution: WB: 1:500-1:2000
Target: Histone H3.3
Purification: Immunogen affinity purified
Reactivity: Human, Mouse, Rat