DDX21 antibody - 100 µg
Host : Rabbit
Clonality: Polyclonal
Clone:
Isotype: IgG
Immunogen: DEAD(Asp-Glu-Ala-Asp) box polypeptide 21
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight: 87 kDa
Uniprot: Q9NR30
Gene id: 9188
Background: RNA helicase that acts as a sensor of the transcriptional status of both RNA polymerase(Pol) I and II: promotes ribosomal RNA(rRNA) processing and transcription from polymerase II(Pol II)(PubMed:25470060). Binds various RNAs, such as rRNAs, snoRNAs, 7SK and, at lower extent, mRNAs(PubMed:25470060). In the nucleolus, localizes to rDNA locus, where it directly binds rRNAs and snoRNAs, and promotes rRNA transcription, processing and modification. Required for rRNA 2'-O-methylation, possibly by promoting the recruitment of late-acting snoRNAs SNORD56 and SNORD58 with pre-ribosomal complexes(PubMed:25470060, PubMed:25477391). In the nucleoplasm, binds 7SK RNA and is recruited to the promoters of Pol II-transcribed genes: acts by facilitating the release of P-TEFb from inhibitory 7SK snRNP in a manner that is dependent on its helicase activity, thereby promoting transcription of its target genes(PubMed:25470060). Functions as cofactor for JUN-activated transcription: required for phosphorylation of JUN at 'Ser-77'(PubMed:11823437, PubMed:25260534). Can unwind double-stranded RNA(helicase) and can fold or introduce a secondary structure to a single-stranded RNA(foldase)(PubMed:9461305). Involved in rRNA processing(PubMed:14559904, PubMed:18180292).
Field of research: Metabolism
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, WB, IHC, IF, IP
Dilution: WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200
Target: DDX21
Purification: Immunogen affinity purified
Reactivity: Human, Mouse, Rat
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Clonality: Polyclonal
Clone:
Isotype: IgG
Immunogen: DEAD(Asp-Glu-Ala-Asp) box polypeptide 21
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight: 87 kDa
Uniprot: Q9NR30
Gene id: 9188
Background: RNA helicase that acts as a sensor of the transcriptional status of both RNA polymerase(Pol) I and II: promotes ribosomal RNA(rRNA) processing and transcription from polymerase II(Pol II)(PubMed:25470060). Binds various RNAs, such as rRNAs, snoRNAs, 7SK and, at lower extent, mRNAs(PubMed:25470060). In the nucleolus, localizes to rDNA locus, where it directly binds rRNAs and snoRNAs, and promotes rRNA transcription, processing and modification. Required for rRNA 2'-O-methylation, possibly by promoting the recruitment of late-acting snoRNAs SNORD56 and SNORD58 with pre-ribosomal complexes(PubMed:25470060, PubMed:25477391). In the nucleoplasm, binds 7SK RNA and is recruited to the promoters of Pol II-transcribed genes: acts by facilitating the release of P-TEFb from inhibitory 7SK snRNP in a manner that is dependent on its helicase activity, thereby promoting transcription of its target genes(PubMed:25470060). Functions as cofactor for JUN-activated transcription: required for phosphorylation of JUN at 'Ser-77'(PubMed:11823437, PubMed:25260534). Can unwind double-stranded RNA(helicase) and can fold or introduce a secondary structure to a single-stranded RNA(foldase)(PubMed:9461305). Involved in rRNA processing(PubMed:14559904, PubMed:18180292).
Field of research: Metabolism
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, WB, IHC, IF, IP
Dilution: WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200
Target: DDX21
Purification: Immunogen affinity purified
Reactivity: Human, Mouse, Rat