Acetylated tubulin (Lys40) antibody - 100 µg
Host : Mouse
Clonality: Monoclonal
Clone: 6A10
Isotype: IgG1
Immunogen: tubulin, alpha 1a
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight: 52 kDa
Uniprot: Q71U36
Gene id: 7846
Background: Tubulin, composed of heterodimers of alpha and beta tubulin, is the mainly component of microtubules which play important roles in cell motility, mitosis, and intracellular vesicle transport. Both alpha and beta tubulin undergo several posttranslational modifications such as polyglutamylation and acetylation
deacetylation. Tubulin acetylation occurs on lysine-40 at the N-terminal of alpha tubulin and is conserved across species. The histone deacetylase HDAC6 and SIRT2 has been identified as tubulin deacetylases. Reversible acetylation of alpha tubulin may be implicated in regulating microtubule stability, cell motility, and axon regeneration. The level of acetylated tubulin has been linked to epithelial malignancies and sensitivity to chemotherapy. In addition, acetylated tubulin has been widely used as a marker for primary cilia. This antibody is specific to the acetylated tubulin; it does not recognize non-acetylated tubulin.
Field of research: Neuroscience, Cell Division and Proliferation, Metabolism
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, WB, IHC, IF
Dilution: WB: 1:500-1:5000; IF: 1:20-1:200; IHC: 1:20-1:200
Target: Acetylated tubulin (Lys40)
Purification: Protein A+G purification
Reactivity: Human, Mouse, Rat, Dog
Clonality: Monoclonal
Clone: 6A10
Isotype: IgG1
Immunogen: tubulin, alpha 1a
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight: 52 kDa
Uniprot: Q71U36
Gene id: 7846
Background: Tubulin, composed of heterodimers of alpha and beta tubulin, is the mainly component of microtubules which play important roles in cell motility, mitosis, and intracellular vesicle transport. Both alpha and beta tubulin undergo several posttranslational modifications such as polyglutamylation and acetylation
deacetylation. Tubulin acetylation occurs on lysine-40 at the N-terminal of alpha tubulin and is conserved across species. The histone deacetylase HDAC6 and SIRT2 has been identified as tubulin deacetylases. Reversible acetylation of alpha tubulin may be implicated in regulating microtubule stability, cell motility, and axon regeneration. The level of acetylated tubulin has been linked to epithelial malignancies and sensitivity to chemotherapy. In addition, acetylated tubulin has been widely used as a marker for primary cilia. This antibody is specific to the acetylated tubulin; it does not recognize non-acetylated tubulin.
Field of research: Neuroscience, Cell Division and Proliferation, Metabolism
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, WB, IHC, IF
Dilution: WB: 1:500-1:5000; IF: 1:20-1:200; IHC: 1:20-1:200
Target: Acetylated tubulin (Lys40)
Purification: Protein A+G purification
Reactivity: Human, Mouse, Rat, Dog